H. Modjtahedi et al., PHASE-I TRIAL AND TUMOR-LOCALIZATION OF THE ANTI-EGFR MONOCLONAL-ANTIBODY ICR62 IN HEAD AND NECK OR LUNG-CANCER, British Journal of Cancer, 73(2), 1996, pp. 228-235
The purpose of this study was to determine the effect of the first rat
monoclonal antibody (MAb ICR62) to the epidermal growth factor recept
or (EGFR) in a phase I clinical trial in patients with unresectable sq
uamous cell carcinomas. This antibody effectively blocks the binding o
f EGF, transforming growth factor (TGF)-alpha and HB-EGF to the EGFR,
inhibits the growth in vitro of tumour cell lines which overexpress th
e EGFR and eradicates such tumours when grown as xenografts in athymic
mice. Eleven patients with squamous cell carcinoma of the head and ne
ck and nine patients with squamous cell carcinoma of the lung, whose r
umours expressed EGFR, were recruited. Groups of three patients were t
reated with 2.5 mg, 10 mg, 20 mg or 40 mg of ICR62 and a further eight
patients received 100 mg. All patients were evaluated for toxicity us
ing WHO criteria. Patients' sera were tested for the clearance of MAb
ICR62 and the development of human anti-rat antibodies (HARA). No seri
ous (WHO Grade III-IV) toxicity was observed in patients treated with
up to 100 mg of antibody ICR62. Antibody ICR62 could be detected at 4
h and 24 h in the sera of patients treated with 40 mg or 100 mg of ICR
62. Only 4/20 patients showed HARA responses (one at 20 mg, one at 40
mg and two at 100 mg doses) and of these only the former two were anti
-idiotypic responses. In four patients receiving doses of ICR62 at 40
mg or greater, biopsies were obtained from metastatic lesions 24 h lat
er and examined for the localisation of ICR62 using anti-rat antibody
reagent. In these patients we showed the localisation of MAb ICR62 to
the membranes of tumour cells; this appeared to be more prominent at t
he higher dose of 100 mg. On the basis of these data we conclude that
MAb ICR62 can be administered safely to patients with squamous cell ca
rcinomas and that it can localise efficiently to metastases even at re
latively low doses.