N. Utoguchi et al., EFFECT OF TUMOR CELL-CONDITIONED MEDIUM ON ENDOTHELIAL MACROMOLECULARPERMEABILITY AND ITS CORRELATION WITH COLLAGEN, British Journal of Cancer, 73(1), 1996, pp. 24-28
Conditioned medium prepared from mouse melanoma B16 cells (B16-CM) inc
reases the macromolecular permeability of bovine aortic, venous and hu
man umbilical vein endothelial monolayer. Collagen, which is synthesis
ed by endothelial cells, has an important Function in regulating the p
ermeability of endothelial monolayer. Briefly, low collagen content le
ads to hyperpermeable structure of the endothelial monolayer. In the p
resent studies, we examined the relationship between the increase of e
ndothelial permeability and content of synthesised collagen of endothe
lial cells cultured with B16-CM. The B16-CM reduced endothelial collag
en content but did not digest collagen directly. Matrix metalloprotein
ase inhibitor, 1,10-phenanthroline, inhibited the increase in permeabi
lity due to addition of B16-CM. These data suggest that B16-CM acts on
endothelial cells, stimulating the digestion of endothelial collagen,
and that the reduced content of collagen leads to the hyperpermeabili
ty of the endothelial monolayer.