IMMUNOHISTOCHEMICAL LOCALIZATION OF GALANIN IN BOVINE REPRODUCTIVE-ORGANS

The vagina, uterus and oviduct were shown to receive galanin immunorea ctive (GAL-IR) nerve fibres, the number of which varied between partic ular organs. In the ovary, GAL-IR nerves were absent. A small number o f these nerves were located in the layers of the oviduct. A moderate n umber of GAL-IR nerves were situated in the body and uterine horns, wh ereas the uterine cervix and vagina wall contained a large number of G AL-IR nerve fibres, evenly distributed throughout particular membranes of the organs. GAL-IR nerves were found to contain, simultaneously, e ither vasoactive intestinal polypeptide (VIP), substance P (SP) or Leu (5)-enkephalin (ENK). Many of the GAL-IR nerves contained tyrosine hyd roxylase (TH). A group of GAL-TR nerves that did not possess immunorea ctivity to VIP, SP, ENK or TH was also observed.