EFFECT OF SELENIUM ON ISCHEMIC AND REPERFUSION INJURY IN ISOLATED GUINEA-PIG LUNGS

1. An experimental comparative study on isolated guinea pig lungs was carried out to determine the effect of selenium added to pulmoplegic s olution on ischemic lung preservation. 2. Two different types of solut ions (Eurocollins in control group and Eurocollins + selenium 10(-3) M in experimental group) were infused before 3 hr of normothermic ische mia. 3. Tissue malone dialdehyde (MDA) and tissue glutathione (GSH) le vels were assessed before the ischemic period, after the ischemia and at the end of reperfusion. Electron microscopic changes were also stud ied at the end of reperfusion to compare the cellular injury between t he groups. 4. Addition of selenium before the ischemic period relative ly decreased tissue MDA levels after reperfusion but did not alter tis sue GSH levels.