Le. Rikans et al., LOSS OF MITOCHONDRIAL-MEMBRANE POTENTIAL IS NOT ESSENTIAL TO HEPATOCYTE KILLING BY ALLYL ALCOHOL, Toxicology letters, 81(2-3), 1995, pp. 159-165
Allyl alcohol-induced LDH leakage from isolated rat hepatocytes was pr
eceded by a decrease in rhodamine 123 retention, signifying a loss of
mitochondrial membrane potential. Addition of dithiothreitol (DTT) pre
vented the drop in membrane potential and completely prevented cell ki
lling by allyl alcohol. In contrast, cyclosporin A and trifluoperazine
delayed the loss of membrane potential without affecting cytolethalit
y. The results indicate that a drop in mitochondrial membrane potentia
l is not essential for allyl alcohol lethality. The mitochondrial dysf
unction produced by allyl alcohol appears to be the consequence of an
earlier event in the toxicity that is reversible by DTT.