A. Kolman et M. Dusinska, COMPARISON OF PROPYLENE-OXIDE AND EPICHLOROHYDRIN EFFECTS IN 2 TRANSFORMATION TESTS (C3H 10T1/2 AND SHE CELLS)/, Toxicology letters, 81(2-3), 1995, pp. 213-221
The neoplastic cell transformation induced by propylene oxide (PO) and
epichlorohydrin (ECH) was studied in two in vitro assays, mouse embry
o fibroblasts (C3H/10T1/2) and Syrian hamster embryo (SHE) cells. In C
3H/10T1/2 cells treated with PO (2.5-10 mM), the transformation freque
ncies were enhanced about 2-4 times in the presence of 12-O-tetradecan
oylphornol-13-acetate (TPA), compared with the transformation frequenc
ies in the absence of TPA. In SHE cells, an even higher increase (abou
t 6-9 times) was reached at concentrations of 2.5-20 mM. The presence
of TPA strongly influenced the ability of ECH to induce the morphologi
cal transformation at low-moderate concentrations (0.25-1 mM). At the
highest concentrations applied, 1 mM in C3H/10T1/2 cells and 0.5 mM in
SHE cells, 41- and 4-fold increases, respectively, were observed. In
C3H/10T1/2 cells, the rad-equivalence (rad/mMh) of PO and ECH in the p
resence of TPA was calculated to be 36 +/- 8 and 296 +/- 65 (mean +/-
S.E.), respectively.