INTEGRATION SITE OF HUMAN PAPILLOMAVIRUS TYPE-18 DNA IN CHROMOSOME BAND-8Q22.1 OF C4-1 CERVICAL-CARCINOMA - DNASE-I HYPERSENSITIVITY AND METHYLATION OF CELLULAR FLANKING SEQUENCES

The C4-1 cell line derived from a non-keratinizing squamous cell carci noma of the uterine cervix contains integrated human papillomavirus-18 DNA. Fluorescence in situ hybridization of C4-1 cells demonstrated a single viral integration site at 8q22.1 on a derivative chromosome ori ginating from an 8q;12q translocation. 8q22 is a site of chromosome fr agility and is also recombinogenic in several human malignancies. DNas e I hypersensitivity of the integration site was studied with a cellul ar flanking probe. A hypersensitive site was detected within 3 kb from viral DNA. The integration sites are undermethylated in C4-1 and HeLa cells and fully methylated in tumor cell lines of other origin, such as lymphoid cells. (C) 1994 Wiley-Uss, Inc.