CHARACTERIZATION BY WESTERN BLOTTING OF MOUSE INTESTINAL GLYCOPROTEINS BOUND BY ESCHERICHIA-COLI HEAT-LABILE ENTEROTOXIN TYPE-I

Escherichia coli heat-labile enterotoxin type I (LT-I)-binding galacto proteins, which were not recognized by cholera toxin, were detected in intestinal epithelial cells of BALB/c mouse by Western blotting. Inhi bitory studies using lectins and modifications of sugar chain suggest that LT-I recognizes certain mucin-type sugar chains containing the te rminal Gal beta 1-3GalNAc sugar sequence in the galactoproteins. The t erminal sugar sequence is identical to that of GM1 ganglioside, the we ll-documented functional receptor for LT-I.