C. Legrand et al., COMPARISON OF METABOLISM OF HYBRIDOMA CELLS CULTURED IN MEDIA SUPPLEMENTED WITH WHEY OR FETAL CALF SERUM, Biotechnology progress, 9(6), 1993, pp. 573-579
The effects of industrial whey on cell physiology were studied in a re
peated fed-harvest mode using free-suspended murine hybridoma cells. A
fter several days of culture in medium containing 9% whey and 1% fetal
calf serum (FCS), cell growth and viability, carbohydrate, amino acid
, energy metabolism, and antibody production rates were investigated.
Differences were found between cells cultured in whey medium and those
cultured in conventional FCS medium. The cell growth obtained in medi
um supplemented with fresh whey was similar to that obtained in FCS me
dium. The viability showed an increase for hybridoma cells cultured in
whey medium. Glucose consumption rates were similar, whereas the lact
ate production rate was higher in whey medium. The metabolic uptake ra
tes of glutamine and ammonia increased in whey medium. More alanine, g
lutamate, glycine, and proline were produced; their production partly
came from glutamine and lactate. The consumption rates of branched ami
no acids changed little; their utilization was higher in whey medium.
Finally, antibody productivity was increased about 20% for cells cultu
red in medium containing whey.