SYNTHESIS OF HIC-PACKING AND STUDY OF ITS CHROMATOGRAPHIC CHARACTERISTICS

A HIC support has been synthesized by using the domestic silica with m ajor pore diameter, The mixture of six standard proteins, alpha-Amylas e in saliva and rIFN-gamma from E. Coli, were separated through gradie nt elution by high-performance hydrophobic interaction chromatography respectively. The chromatographic column has less irreversible adsorpt ion. alpha-Amylase and lysozyme used in this study are almost quantita tively recovered on this column, The effects of salt concentration in eluent and temperature on retention behaviours of proteins were invest igated with packing prepared, and the chromatographic attribution of t he support has been demonstrated.