RECLASSIFICATION OF PAENIBACILLUS (FORMERLY BACILLUS) PULVIFACIENS (NAKAMURA 1984) ASH ET-AL 1994, A LATER SUBJECTIVE SYNONYM OF PAENIBACILLUS (FORMERLY BACILLUS) LARVAE (WHITE 1906) ASH ET-AL 1994, AS A SUBSPECIES OF PAENIBACILLUS-LARVAE, WITH EMENDED DESCRIPTIONS OF PAENIBACILLUS-LARVAE AS PAENIBACILLUS-LARVAE SUBSP LARVAE AND PAENIBACILLUS-LARVAE SUBSP PULVIFACIENS
M. Heyndrickx et al., RECLASSIFICATION OF PAENIBACILLUS (FORMERLY BACILLUS) PULVIFACIENS (NAKAMURA 1984) ASH ET-AL 1994, A LATER SUBJECTIVE SYNONYM OF PAENIBACILLUS (FORMERLY BACILLUS) LARVAE (WHITE 1906) ASH ET-AL 1994, AS A SUBSPECIES OF PAENIBACILLUS-LARVAE, WITH EMENDED DESCRIPTIONS OF PAENIBACILLUS-LARVAE AS PAENIBACILLUS-LARVAE SUBSP LARVAE AND PAENIBACILLUS-LARVAE SUBSP PULVIFACIENS, International journal of systematic bacteriology, 46(1), 1996, pp. 270-279
A polyphasic taxonomic study of four strains of Paenibacillus larvae a
nd four strains of Paenibacillus pulvifaciens (including duplicates of
both type strains) supported the reclassification of both former Baci
llus species into one species, P, larvae, Our conclusions were based o
n morphological and Analytab Products (API) tests, sodium dodecyl sulf
ate-polyacrylamide gel electrophoresis (SDS-PAGE) of whole-cell protei
ns, gas chromatography of methylated fatty acids, pyrolysis mass spect
rometry, DNA-DNA binding, and the following genomic fingerprinting met
hods: amplified ribosomal DNA restriction analysis, random amplified p
olymorphic DNA analysis, and AFLP analysis, The last method is a novel
high-resolution DNA fingerprinting technique based on the selective a
mplification of restriction fragments, Despite more than 90% DNA relat
edness between the strains studied, SDS-PAGE of whole-cell proteins, b
iochemical tests, and DNA fingerprinting (AFLP) distinguished between
the P. larvae and P, pulvifaciens strains at the subspecies level, Tak
ing this evidence along with differences in pathogenicity, we propose
to reclassify the honeybee pathogens P, larvae and P. pulvifaciens as
P, larvae subsp, larvae and P, larvae subsp, pulvifaciens.;in emended
description of the species and descriptions of the subspecies are give
n, The type strains are P, larvae subsp, larvae ATCC 9545 (LMG 9820) a
nd P, larvae subsp, pulvifaciens NRRL B-3685 (LMG 6911 and;ATCC 13537)
.