N. Pascual et al., QUANTIFICATION OF ECDYSTEROIDS BY IMMUNOASSAY - COMPARISON OF ENZYME-IMMUNOASSAY AND RADIOIMMUNOASSAY, Zeitschrift fur Naturforschung. C, A journal of biosciences, 50(11-12), 1995, pp. 862-867
The performance of enzyme immunoassay (EIA) and radioimmunoassay (RTA)
in the quantitative analysis of ecdysteroids was compared. The EIA wa
s found to be at least equivalent to the RIA with respect to analytica
l range and sensitivity and to be more comfortable with respect to saf
ety and time saving. When biological samples were analyzed by both ass
ays a good correlation (r = 0.83) was found. Since the EIA has certain
advantages over the RIA, we now recommend the use of the former assay
for the quantification of ecdysteroids.