A NOVEL EXPRESSION SYSTEM FOR SALMONELLA-TYPHIMURIUM ALLOWING HIGH PRODUCTION LEVELS, PRODUCT SECRETION AND EFFICIENT RECOVERY

A novel expression system for heterologous production in Salmonella ty phimurium, taking advantage of the promoter, signal sequence and two I gG-binding domains (ZZ) from staphylococcal protein A, has been invest igated. The production of two different fusions proteins, ZZ-M3 and ZZ -MS, was characterized in terms of production levels. product localiza tion (periplasm or culture medium) and product quality after affinity purification. High expression levels and efficient product secretion w ere obtained, making the system attractive for vaccine development. Th e potential use of S. typhimurium as host for heterologous production in biotechnology is discussed. (C) 1996 Academic Press, Inc.