MICRONUCLEI INDUCTION BY CARBOPLATIN IN HUMAN LYMPHOCYTE SUBPOPULATIONS

Micronuclei (MN) induction by carboplatin, cis-diammine-1,1-cyclobutan e decarboxylate platinum (II) (CBDCA), in B and T lymphocytes was stud ied by the MAC (morphology/antibody/chromosome) method which allows th e immunologic identification of different cell lineages. An increased frequency of MN in B and T lymphocytes in CBDCA-treated cultures compa red with controls was observed (p<0.001). CBD cells were found to be m ore sensitive to CBDCA damage. CBDCA-treated cultures showed a decreas e, albeit statistically non-significant, in the proportion of CBD inte rphasic and mitotic cells. Furthermore, higher MN frequencies in isola ted lymphocytes than in whole blood in both control and CBCDA-treated cultures were observed.