K. Kashihara et al., LEVODOPA INDUCES AP-1 AND CREB DNA-BINDING ACTIVITIES IN THE RAT STRIATUM, PSYCHIATRY AND CLINICAL NEUROSCIENCES, 49(5-6), 1995, pp. 291-294
In order to elucidate the effect of levodopa and bromocriptine on the
DNA-binding activities of transcription factors, AP-1 and CREB DNA-bin
ding activities were investigated using gel-shift assay. Intraperitone
al administration of 100 mg/kg levodopa with 50 mg/kg benserazide in r
ats increased both AP-1 and CREB DNA-binding activities in the dorsola
teral aspect of the striatum. The major proteins composing the increas
ed AP-1 were JunB and JunD. Bromocriptine at doses of 2.5 and 5.0 mg/k
g, however, did not increase these binding activities. Present results
suggest that levodopa but not bromocriptine induces these transcripti
on-regulating proteins in the striatum with normal dopaminergic functi
oning.