The sequences of 10 conservative regions (CR) of minicircles of 6 sele
cted isolates of freshwater fish trypanosomes have typical organizatio
n of this region with high degree of sequence conservation. The compar
ison with CRs of other trypanosomatids showed that freshwater fish try
panosomes represent a compact separate group within the Trypanosoma. T
he alignment of all sequences obtained revealed, however, the existenc
e of 2 types of CRs in sequenced minicircles, with the differences con
centrated in a short region. Taxonomic consequences of these results a
re discussed.