BIOSENSORS BASED ON CROSS-LINKING OF BIOTINYLATED GLUCOSE-OXIDASE BY AVIDIN

Avidin, having four biotin binding units, cross-links and immobilizes glucose oxidase (GOX) labeled with multiple biotins (B-GOX) when solut ions of B-GOX and avidin are mixed on an electrode. The H2O2 flux gene rated in the B-GOX catalyzed oxidation of glucose by dissolved O-2 is measured as an electroreduction current at a horseradish peroxidase (H RP) redox connducting hydrogel electrode poised at + 100 mV (Ag/AgCl). The sensitivity of the resulting glucose sensor is 0.14 A cm(-2) M(-1 ) with a linear range up to 2mM glucose.