GENERATION OF A MURINE MONOCLONAL-ANTIBODY SPECIFIC FOR N-GLYCOLYLNEURAMINIC ACID-CONTAINING GANGLIOSIDES THAT ALSO RECOGNIZES SULFATED GLYCOLIPIDS

Authors
VAZQUEZ AM ALFONSO M LANNE B KARLSSON KA CARR A BARROSO O FERNANDEZ LE RENGIFO E LANIO ME ALVAREZ C ZEUTHEN J PEREZ R
Citation
Am. Vazquez et al., GENERATION OF A MURINE MONOCLONAL-ANTIBODY SPECIFIC FOR N-GLYCOLYLNEURAMINIC ACID-CONTAINING GANGLIOSIDES THAT ALSO RECOGNIZES SULFATED GLYCOLIPIDS, Hybridoma, 14(6), 1995, pp. 551-556
Citations number
39
Categorie Soggetti
Immunology
Journal title
Hybridoma → ACNP
ISSN journal
0272457X
Volume
14
Issue
6
Year of publication
1995
Pages
551 - 556
Database
ISI
SICI code
0272-457X(1995)14:6<551:GOAMMS>2.0.ZU;2-Q
Abstract
The P3 murine monoclonal antibody (MAb) was generated by immunizing BA LB/c mice with NeuGcGM3 included into liposomes. The specificity of th is MAb was defined by an enzyme-linked immunosorbent assay and immunos taining on thin-layer chromatograms. P3 MAb binds to NeuGc-containing gangliosides and was shown also to react with sulfated glycolipids. A preliminary immunohistochemical study showed that the P3 MAb was able to recognize antigens expressed in human breast tumors.