Between October 1991 and February 1992, 41 of 53 known adult and subad
ult Ethiopian wolves (Canis simensis) in five adjacent packs in the Ba
le Mountains National Park, Ethiopia, died or disappeared. Brain smear
s from two carcasses were positive for rabies by the immunofluorescenc
e test, and rabies virus was isolated from the brains by mouse inocula
tion. Based on monoclonal antibody tests on the mouse brains, we ident
ified the virus as a minor variant of the serotype 1 rabies viruses fo
und in domestic dogs and wild canids of Africa. Sera from two of 15 Et
hiopian wolves had rabies virus neutralizing antibody.