BIOMASS ESTIMATION OF ASPERGILLUS-NIGER GROWING ON REAL AND MODEL SUPPORTS IN SOLID-STATE FERMENTATION

Direct hydrolysis of Aspergillus niger mycelium growth on amberlite IR A-900 or sugar cane bagasse on solid state fermentation followed by th e analysis of soluble protein by the dye binding method was carried ou t. Hydrolysis with phosphoric acid 0.25M during 7 min allowed maxima p rotein extraction available to be measured. Color interference of medi um components was not observed, allowing the use of this method for bi omass estimation when amberlite IRA-900 or sugar cane bagasse are used as support in solid state fermentation processes.