M. Conde et al., RESPIRATORY BURST IS DECREASED BY HUMAN HYPERLIPEMIC SERUM IN RAT PERITONEAL-MACROPHAGES, Free radical research, 24(2), 1996, pp. 107-114
The effect of hyperlipemic human serum on superoxide anion (O2(-)) pro
duction by rat peritoneal macrophages was investigated. Phorbol myrist
ate acetate (PMA)-stimulated O2(-) production was inhibited when cells
were preincubated with hyperlipemic human serum. This inhibition was
specifically carried out by a lipid fraction and was dependent on both
cholesterol and triglyceride serum levels. This inhibitory effect was
not exerted by a direct effect on NADPH-oxidase activity, nor by a pu
tative superoxide dismutase activity present in the serum. With human
neutrophils, we observed a decreased mobility of the cytosolic factor
p47-phox to the membrane during the activation process, caused by hype
rlipemic serum. We did not find any effect of hyperlipemic serum on NO
2- production by cultured rat macrophages. These results suggest that
a pathological increase of circulating plasma lipids may be associated
with an impaired inflamatory capacity of macrophages.