PCR-BASED DETECTION OF GENOMIC MARKERS IN CANINE PARVOVIRUS AND FELINE PANLEUKOPENIA VIRUS

The NS1 and VP1/VP2 genes of canine parvovirus and feline panleukopeni a virus were amplified by the polymerase chain reaction (PCR). The res triction cleavage patterns of the amplified DNA fragments were compare d among these parvoviruses including vaccine strains. Differences of t he restriction site in the NS1 portions were observed between the vacc ine strain and the wild type strain as well as between canine parvovir uses and feline panleukopenia viruses. The restriction patterns of fel ine panleukopenia viruses were distinct from those of canine parvoviru ses, and showed differences between the vaccine strain and its wild ty pe strain. This PCR-based restriction cleavage can be used for ecologi cal study of such viruses.