INDUCTION OF CYTOKINE MESSENGER-RNA BY HE RPES-SIMPLEX VIRUS-INFECTION IN MICE

The induction of the cytokine mRNA after infection with Herpes simplex virus (HSV) was studied using RT-PCR method capable of detecting low levels of mRNA. Total RNA was prepared from spleen lymphocytes 3 h aft er infection with HSV-1 (+GC virulent variant and -GCr attenuated vari ant of Miyama strain) by acid guanidinium thiocyanate-phenol-chlorofor m (AGPC) method. cDNA was synthesized by M-MLV reverse transcriptase, and amplified using the specific oligonucleotide primers for IL-1, IL- 2, IL-3, IL-4, IL-5, IL,-6, TNF-alpha, IFN-alpha, IFN-beta and IFN-gam ma by PCR method. After HSV-1 infection, IFN-alpha, IFN-beta, IFN-gamm a, IL-1beta, IL-4, IL-6 and TNF-alpha mRNA were significantly induced, but IL-2, IL-3 and IL-5 mRNA were not induced. Although IFN-alpha, IF N-gamma and IL-6 mRNA were more strongly induced by infection with +GC virulent variant than -GCr attenuated variant, there was no significa nt difference in the expression of other cytokine mRNA between two var iants. These results demonstrate that cytokine mRNA in addition to IFN was induced by HSV infection, and suggest that cytokines as well as I FNs may play a role in the defense mechanism against HSV infection.