ISOLATION AND CHARACTERIZATION OF CONOPEPTIDES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY COMBINED WITH MASS-SPECTROMETRY AND TANDEM MASS-SPECTROMETRY

Authors
JONES A BINGHAM JP GEHRMANN J BOND T LOUGHNAN M ATKINS A LEWIS RJ ALEWOOD PF
Citation
A. Jones et al., ISOLATION AND CHARACTERIZATION OF CONOPEPTIDES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY COMBINED WITH MASS-SPECTROMETRY AND TANDEM MASS-SPECTROMETRY, Rapid communications in mass spectrometry, 10(1), 1996, pp. 138-143
Citations number
20
Categorie Soggetti
Spectroscopy,"Chemistry Analytical
Journal title
Rapid communications in mass spectrometry → ACNP
ISSN journal
09514198
Volume
10
Issue
1
Year of publication
1996
Pages
138 - 143
Database
ISI
SICI code
0951-4198(1996)10:1<138:IACOCB>2.0.ZU;2-O
Abstract
The sensitivity of mass spectrometry combined with the separatory powe r of high-performance liquid chromatography was used to investigate th e venom of individual cone shells, This analysis has revealed that con e venoms contain a complex mixture of peptides which vary quantitative ly and qualitatively both between and within species, A differential a lkylation procedure followed by tandem mass spectrometric analysis can be used to determine the disulfide bond connectivity in these small, cysteine-rich peptides.