Ajg. Mank et al., DIODE LASER-BASED DETECTION IN LIQUID-CHROMATOGRAPHY AND CAPILLARY ELECTROPHORESIS, TrAC. Trends in analytical chemistry, 15(1), 1996, pp. 1-11
Detection techniques involving diode lasers are increasingly of intere
st in separation science. Diode lasers are small and inexpensive and h
ave a very stable output. However, diode lasers emitting at wavelength
s shorter than 635 nm are not commercially available. This seriously l
imits the applicability of direct detection, since few analytes absorb
at such long wavelengths. Because devices that emit at much shorter w
avelengths are not expected to become available in the near future, in
direct detection schemes involving diode lasers are being developed. T
hese are often based on derivatization with redabsorbing labels and su
bsequent separation of the label and the various derivatized analytes
using either liquid chromatography (LC) or capillary electrophoresis (
CE). The application of diode-laser-based detection in CE is of partic
ular interest because the usual detection methods, e.g., absorption de
tection, do not have the sensitivity required for many applications.