EFFECT OF OILS AND SURFACTANTS ON PULLULAN PRODUCTION RELATIVE TO NITROGEN-SOURCE

The effect of selected vegetable oils, fatty acids and surfactants on pullulan production by Aureobasidium pullulans ATCC 42023 relative to nitrogen source was studied in a corn syrup-based medium. All vegetabl e oils, fatty acids and surfactants screened elevated pullulan product ion by strain ATCC 42023 when ammonium sulphate was the nitrogen sourc e while adding olive oil, oleic acid or Antifoam surfactants into the medium increased pullulan production when corn steep liquor served as the nitrogen source. The pullulan content of the polysaccharide elabor ated by ammonium sulphate-grown cells on day 3 varied from 100% when o live oil, Antifoam 204 or Antifoam 289 was added to similar to 50% if linoleic or oleic acid was present. The pullulan content of the polysa ccharide produced by corn steep liquor-grown cells ranged from 80% if supplemented with sunflower oil to 36% when oleic acid was included.