CLONING OF THE MOUSE GENE ENCODING PLASMA GLUTATHIONE-PEROXIDASE - ORGANIZATION, SEQUENCE AND CHROMOSOMAL LOCALIZATION

Using a reverse transcription coupled to PCR amplification strategy, w ith degenerated primers localized in highly conserved domains of known glutathione peroxidase (GPX) proteins, we have generated, from mouse epididymal RNA, a cDNA fragment which was subsequently used to isolate a genomic clone encoding mouse plasma GPX (GPX3). GPX3 is a major enz yme in reducing lipid hydroperoxides and hydrogen peroxide in plasma. We confirm here that the mouse epididymis is a new site of expression of GPX3 and report, together with the sequence, the structural analysi s and the chromosomal localization of the mouse GPX3 single-copy gene to chromosome 11.