Gl. Yount et al., CELL-CYCLE SYNCHRONY UNMASKS THE INFLUENCE OF P53 FUNCTION ON RADIOSENSITIVITY OF HUMAN GLIOBLASTOMA CELLS, Cancer research, 56(3), 1996, pp. 500-506
Although ionizing radiation causes DNA damage that can play a role in
tumorigenesis, such irradiation is also an important modality of cance
r therapy, We studied the radiation response of the U-87 MG human glio
blastoma cell line and transfected derivatives in which p53 function h
ad been inactivated, Although little effect of p53 on the radiation se
nsitivity of asynchronously growing cultures could be detected, inacti
vation of p53 resulted in a large increase in clonogenic survival when
cells synchronized by mitotic selection were irradiated in early G(1)
. The radiation dose sufficient to reduce cellular clonogenicity by 1
log in cells expressing functional p53 was 3.26 +/- 0.12 Gy, whereas a
much higher dose (7.41 +/- 0.44 Gy) was required to achieve the same
killing effect in cells in which p53 was inactivated. Apoptosis was ex
cluded as a probable mechanism contributing to the radiosensitivity of
these cells. Fluorescence-activated cell sorter analysis, continuous
labeling with tritiated thymidine, and time-lapse videomicroscopy docu
mented the first example of a prolonged p53-dependent G(1) arrest indu
ced by ionizing radiation during the first postirradiation cell cycle
of tumor cells, suggesting a role for G(1) arrest in determining the s
ensitivity of these cells to irradiation.