Vn. Verbenko et Vl. Kalinin, INCREASE IN BACTERIOPHAGE RADIATION-RESIS TANCE AS A RESULT OF ENHANCED EXPRESSION OF STRESS SYSTEMS IN HOST-CELLS, Genetika, 31(12), 1995, pp. 1630-1636
By means of polyacrylamide gel electrophoresis (PAGE) of proteins from
radiation-resistant Gam(r) mutants of Escherichia coli, it was shown
that induction and elimination of RecA protein in these mutants are ki
netically more rapid than in wild type cells, and heat-shock proteins
(HSP) are hyperproduced even at a normal temperature (32 degrees C). g
amma- and UV-irradiated bacteriophages were used to study the results
of simultaneous enhanced expression of two stress repair systems. Radi
ation-resistant mutants are similar to wild type cells in their abilit
y to reactivate phages lambda CI, phi 80 vir, and T 4D inactivated by
gamma-rays and UV-light. W-reactivation of gamma-irradiated phages lam
bda and 80 vir is respectively 1.5 and 1.2 times higher in Gam(r) cell
s in which maximal w-reactivation was observed at wide range of doses
(from 300 to 2000 Gy) whereas in wild type cells the peak of W-reactiv
ation was registered at doses of 400 to 450 Gy. The phage lambda gamma
-, irradiated upon adsorption on the cells of a radiation-resistant mu
tant, was two times more resistant to gamma-rays (DMF = 2 at LD(10)) t
han when irradiated upon adsorption on wild type cells. Postirradiatio
n degradation of the phage lambda DNA, when irradiated within Gam(r) c
ells, was significantly lower than in wild type cells, and preirradiat
ion of the cells decreased phage DNA degradation (12% in Gam(r) cells
and 30% in wild-type cells). The role of an increased HSP level and ex
pression of SOS-regulon in radiation resistance and possible interacti
on of stress systems in bacterial cells are discussed.