DISTRIBUTION OF ALLELES OF THE KAPPA-CASE IN GENE IN POPULATIONS OF EUROPEAN BISON (BISON-BONASUS)

The polymerase chain reaction method, followed by restriction fragment length polymorphism analysis (PCR-RFLP), was used to study the allele s of the kappa-casein gene in a sample of European bison (n = 38). On the basis of the distribution of the restriction sites, a new allele k appa-CnG was revealed in the European bison, in addition to the kappa- CnA allele represented in cattle. The nucleotide sequence of an exon 4 fragment of the kappa-CnG allele was obtained. In the population of E uropean bison alleles kappa-CnA and kappa-CnG occurred at frequencies 0.224 and 0.776, respectively.