SECRETORY GRANULE PROTEINS FROM THE SUBVENTRAL ESOPHAGEAL GLANDS OF THE POTATO CYST-NEMATODE IDENTIFIED BY MONOCLONAL-ANTIBODIES TO A PROTEIN-FRACTION FROM 2ND-STAGE JUVENILES

Sodium dodecyl sulfate-extracted proteins from second-stage juveniles (J(2)) of the potato cyst nematode Globodera rostochiensis were fracti onated by preparative continuous flow electrophoresis, and monoclonal antibodies (MAbs) were raised against the 38- to 40.5-kDa protein frac tion, Screening of the hybridoma culture fluids by immunofluorescence microscopy of J(2) resulted in the identification of 12 MAbs that boun d specifically to the subventral esophageal glands, On Western blots o f J(2) these MAbs identified four protein bands with apparent molecula r masses of 30, 31, 39, and 49 kDa, Immunoelectron microscopy with one of these MAbs showed an intense labeling of the electron dense core o f the secretory granules in the subventral gland cells of J(2). It is concluded that one or more of these proteins are localized within thes e secretory granules, Immunofluorescence microscopy of J(2) from other plant parasitic nematode species showed that most of these MAbs also bind to the subventral glands of G. pallida and G. tabacum but not of Heterodera schachtii, H. glycines, Meloidogyne incognita, or M, hapla.