CD14(-COLONY-STIMULATING FACTOR (G-CSF)-MOBILIZED PERIPHERAL-BLOOD MONONUCLEAR-CELLS INDUCE SECRETION OF INTERLEUKIN-6 AND G-CSF BY MARROW STROMA() CELLS IN GRANULOCYTE)
M. Mielcarek et al., CD14(-COLONY-STIMULATING FACTOR (G-CSF)-MOBILIZED PERIPHERAL-BLOOD MONONUCLEAR-CELLS INDUCE SECRETION OF INTERLEUKIN-6 AND G-CSF BY MARROW STROMA() CELLS IN GRANULOCYTE), Blood, 87(2), 1996, pp. 574-580
The ability of granulocyte colony-stimulating factor (G-CSF)-mobilized
peripheral blood mononuclear cells (G-PBMCs) to induce secretion of c
ytokines in primary longterm marrow cultures (LTC) or in the human mar
row stromal cell line HS23 was compared with that of marrow mononuclea
r cells. Equal numbers of G-PBMCs or marrow mononuclear cells were add
ed to stromal cultures, supernatants were harvested at day 4 and level
s of interleukin-1 alpha (IL-1 alpha), IL-1 beta, IL-2, IL-6, G-CSF, a
nd tumor necrosis factor alpha (TNF alpha) were determined. G-PBMCs in
duced 21.4-fold higher levels of IL-6 and 12.5-fold higher levels of G
-CSF in LTC cocultures compared with marrow mononuclear cells and indu
ced 20.6-fold more IL-6 and 6.3-fold more G-CSF when added to HS23 cel
ls. Experiments using sorted populations of CD20(+), CD3(+), and CD14(
+) cells showed that CD14(+) cells within G-PBMCs were responsible for
triggering the production of IL-6 and G-CSF. The effect did not requi
re cell-cell contact and was inhibited when neutralizing antibodies to
lL-1 alpha and IL-1 beta were used in combination. In these experimen
ts, the greater stimulating ability of G-PBMCs is most likely attribut
able to the greater number of CD14(+) cells in G-PBMCs (26.1% +/- 2.3%
) compared with marrow (2.5% +/- 0.8%), because equal numbers of CD14(
+) cells sorted from marrow and G-PBMCs showed comparable ability to i
nduce IL-6 and G-CSF when placed directly on stromal cells. (C) 1996 b
y The American Society of Hematology.