I. Sahdev et al., INTERLEUKIN-1 PRODUCTION FOLLOWING T-CELL-DEPLETED AND UNMODIFIED MARROW CRAFTS, Pediatric hematology and oncology, 13(1), 1996, pp. 55-67
Interleukin-1 (IL-1) production by endotoxin-stimulated cultured monoc
ytes from 31 participants in grafts of marrow depleted of mature cellu
lar elements by treatment with soybean agglutinin and sheep red blood
cells (SBA(-)E(-)) and 12 recipients of unfractionated bone marrow wer
e studied and compared with normal controls. Patients were studied pri
or to marrow transplant (BMT) and at 1 month, 2 to 4 months, and 5 to
6 months post-transplant. Deficiencies in IL-1 production (<50 units)
were detected in both transplant groups prior to and at 1 month post-B
MT. From 2 to 4 months post-transplant, 67% of the recipients of unmod
ified marrow and 45% of the recipients of SBA(-)E(-) marrow grafts pro
duced a normal level of IL-1. By 5 to 6 months post-transplant and the
reafter, the proportions of patients exhibiting deficiencies in IL-1 p
roduction in each group were equally low. We also evaluated the impact
of early deficiencies of IL-1 on engraftment, hematopoietic function,
and immunological reconstitution. Deficiencies in IL-1 production per
sisting to 2 to 4 months post-BMT did not significantly affect the deg
ree of chimerism or the time to recovery of neutrophil counts to 500/m
u l in recipients of either unmodified or T-cell-depleted marrow. Plat
elet recovery during the first 50 days posttransplant was significantl
y slower in the IL-1-deficient group, but thereafter rebounded, so tha
t by 4 months post-BMT patients nts with initial deficiencies in IL-1
production achieved levels comparable with those attained by patients
with normal production of IL-1. When we looked at the lymphocyte respo
nse to phytohemagglutinin (PHA), there was no difference detected amon
g patients with or without IL-1 deficiency receiving unmodified transp
lants. In contrast, recipients of T-cell-depleted grafts exhibiting a
prolonged deficiency of IL-1 experienced a slower rate of recovery of
PHA responses. Our results suggest that IL-1 may play an important rol
e in the early expansion of megakaryocytic precursors following an all
ogeneic marrow transplant and may facilitate the functional developmen
t of allogeneic lymphoid progenitors following a T-cell-depleted marro
w graft.