THE EFFECT OF CYCLOSPORINE-A AND TRIFLUOPERAZINE ON AMYLASE SECRETIONFROM RAT PAROTID-GLANDS IN-VITRO

In the present study the effects of trifluoperazine (TFP) and/or cyclo sporine A (CsA) on the amylase secretion of rat parotid gland lobules were investigated. Cyclosporine A, in doses of 10(-6), 3 x 10(-6) and 10(-5) M, caused a dose-dependent reduction in amylase secretion. Trif luoperazine, a well-established calmodulin antagonist, also significan tly reduced amylase secretion by the gland, confirming that the secret ion is calmodulin dependent. Concurrent administration of CsA and TFP showed a marked positive interaction in reducing the secretion of amyl ase. Thus, the hypothesis that CsA may have Ca2+/calmodulin antagonist ic properties is supported by the present study.