GLYCOSYLATION AND BIOLOGICAL-ACTIVITY OF CAMPATH-1H EXPRESSED IN DIFFERENT CELL-LINES AND GROWN UNDER DIFFERENT CULTURE CONDITIONS

CAMPATH-1H (where CAMPATH is a trade mark of Wellcome group companies) , a humanized IgG antibody used in the therapy of lymphoma, leukaemia and rheumatoid arthritis, has been expressed in Chinese hamster ovary, YO myeloma and NSO myeloma cell lines, These engineered cell lines we re grown under different culture conditions, and the antibody isolated and purified. N-Linked oligosaccharides, on the CH2 heavy chain regio n of the antibody, were isolated and analysed by hydrazinolysis, high- performance anion-exchange chromatography with pulsed amperometric det ection, laser-desorption mass spectrometry and sequential exoglycosida se treatment, Both the glycosylation pattern and the biological activi ty of CAMPATH-1H, as measured by antibody-dependent cell-mediated cyto toxicity, were markedly affected by the cell line used to express the antibody, It is concluded that glycosylation of the antibody may be im portant in the clinical outcome of therapy.