HIGH-DOSE SELEGILINE AUGMENTS STRIATAL UBIQUINOL IN MOUSE - AN INDICATION OF DECREASED OXIDATIVE STRESS OR OF INTERFERENCE WITH MITOCHONDRIAL RESPIRATION - A PILOT-STUDY
Me. Gotz et al., HIGH-DOSE SELEGILINE AUGMENTS STRIATAL UBIQUINOL IN MOUSE - AN INDICATION OF DECREASED OXIDATIVE STRESS OR OF INTERFERENCE WITH MITOCHONDRIAL RESPIRATION - A PILOT-STUDY, Journal of neural transmission. Supplementum, (46), 1995, pp. 149-156
The effects of unspecific doses of the irreversible monoamine oxidase
inhibitor selegiline on alpha-tocopherol, alpha-tocopherolquinone, ubi
quinol and ubiquinone were studied in frontal cortex, hippocampus and
striatum of male C57BL/6 mice 4h and 96h after a single or six injecti
ons of selegiline (100mg/kg body weight, i.p.), respectively. Inhibiti
on of monoamine oxidase was confirmed by activity measurements of its
isoforms A and B in brain stem nuclei and striatum as well as by deter
mination of striatal levels of dopamine and its major metabolites 3,4-
dihydroxyphenylacetic acid and homovanillic acid. In general, levels o
f alpha-tocopherol were not altered and levels of alpha-tocopherolquin
one were below the detection limit. However, 96h following selegiline,
levels of ubiquinols 9 and 10 were significantly increased, whereas l
evels of ubiquinones 9 and 10 concomitantly decreased in the striatum.
Concentrations of ubiquinols and ubiquinones in frontal cortex and hi
ppocampus were unchanged 96 h following selegiline. These data suggest
that selegiline affects the striatal redox ratio of ubiquinol to ubiq
uinone which is important for cellular antioxidant defense and mitocho
ndrial electron transfer.