NUCLEOTIDE-SEQUENCE OF SALICYLATE HYDROXYLASE GENE AND ITS 5'-FLANKING REGION OF PSEUDOMONAS-PUTIDA KF715

The salicylate hydroxylase, a flavoprotein monooxygenase, catalyzes th e decarboxlative hydroxylation of salicylate to form catechol. Nucleot ide sequence of a salicylate hydroxylase gene and its 5'-flanking regi on in chromosomal DNA of Pseudomonas putida KF715 was analyzed. The sa licylate hydroxylase was encoded in an open reading frame with 1308 ba se pairs which can encode a polypeptide of molecular weight 48 kDa wit h 435 amino acids. The open reading frame was preceded by a putative r ibosome-binding sequence. A predicted amino acid sequence of the salic ylate hydroxylase exhibited 84% identity with corresponding enzyme enc oded in NAH7 plasmid, and 20 to 30% homologies with other similar flav oprotein monooxygenases. A 5'-flanking sequence of the salicylate hydr oxylase gene exhibited extensive homology with promoter and nahR-bindi ng site of sal operon in NAH7 plasmid. (C) 1996 Academic Press, Inc.