ASSESSMENT OF THE INFORMATIVE VALUE OF HE IGHT IMMUNOLOGICAL TESTS INHUMAN BRUCELLOSIS

Assessment of the informative value of 8 immunological tests : sero-ag glutination (Wright and Rose Bengale), indirect immunosorbent assay, c ounter immune electrophoresis, ELISA IgG, IgM and IgA, and particle co unting immunoassay (FACIA) has been performed among the results of ser um of 209 patients. The patients were divided in four groups 71 who al ready had brucellosis, 18 Yersinia infections, 12 Tularemia and 108 fr ee of desease. The informative capacities of a positive result of coun ter immune electrophoresis (Protein antigen Brucellin-INRA Tours-Nouzi lly) is higher than others reactions and can be proposed as a confirma tory test of brucellosis. Among others techniques, 4 were found to be more sensitive :Elisa IgA(se = 97,6) and IgG (se = 90,1), IFI (se = 91 ,5) and Rose Bengale (se = 85,9) and can be proposed as screening test for medical diagnosis or epidemiological survey. Many cross-reactions were observed specially with Yersinia enterocolitica even with new se rological methods.