ANALYSIS OF THIOL-CONTAINING COMPOUNDS IN BIOLOGICAL SAMPLES BY CAPILLARY ZONE ELECTROPHORESIS

A new method for the determination of reduced and oxidized glutathione by using capillary zone electrophoresis was developed and compared wi th the N-(1-pyrenyl)maleimide high-performance liquid chromotography m ethod. The tissue samples were obtained from C57BL/6 mice and homogeni zed in deionized water. The detection wavelength was set at 214 nm. A 50-cm effective length X 75 mu m i.d. uncoated fused silica capillary with a 10 mM phosphate buffer at pH 7.5 was used for the experiment. S amples were introduced by pressure injection for 5 s. The voltage for separation was at 20 kV. Between runs, the capillary was regularly was hed with 0.1 N NaOH and deionized water, followed by reconditioning wi th running buffer, The present method was shown to be reproducible and is convenient in that a derivatization step is not required. The meth od has been used to measure glutathione levels in biological samples a nd the results show good agreement with those obtained by high-perform ance liquid chromotography.