A SIMULTANEOUS DETERMINATION OF EVODIAMIN E AND RUTAECARPINE IN ORIENTAL PHARMACEUTICAL DECOCTIONS CONTAINING EVODIA FRUIT BY ION-PAIR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

A simple and precise method was established for the simultaneous deter mination of evodiamine and rutaecarpine in oriental pharmaceutical dec octions containing Evodia Fruit using high-performance liquid chromato graphy with sodium dodecyl sulfate (SDS) as an ion-pair reagent. Evodi amine and rutaecarpine were eluted within 60 min without interference from co-existing components using an ODS column and a mixture of water -methanol-acetonitrile-SDS-phosphoric acid (600:330:.70:5:0.01, v/v/v/ w/v, PH 5.0) as a mobile phase.