THE EFFECT OF PGE(2) ON GLUCOSE-ABSORPTION AND NET MOVEMENT OF WATER AND ELECTROLYTES IN THE JEJUNAL LOOP IN SHEEP

This study was designed cc determine the effect of prostaglandin E(2) (PGE(2)) on glucose absorption and net movement of water and electroly tes in the jejunal Thiry-Vella loop of sheep. Intraluminal perfusion w as performed at 1 ml/min with isotonic 10 mmol/l glucose solution. Osm olality was adjusted by adding NaCl and outflow solution was collected every 10 min. After a 30-min control period, PGE(2) was applied intra luminally for 30 min at concentrations of 25, 50, 100 and 200 mu M. In the control period, water, sodium and chloride were absorbed, while p otassium and bicarbonate were secreted. PGE(2) reversed the net absorp tion of water, sodium and chloride to net secretions, and this secreto ry response to PGE(2) was concentration-dependent. PGE(2) also increas ed bicarbonate secretion depending on the concentration, while potassi um secretion was not significantly changed by PGE(2). The net glucose absorption was decreased by PGE(2); this inhibitory response to PGE(2) was not concentration-dependent. In conclusion, these data suggest th at PGE(2) stimulates the jejunal secretion of water and electrolytes, and inhibits glucose absorption in the jejunal loop of sheep.