Karyometrical analysis of conventional Giemsa-stained mitotic chromoso
mes of parthenitae of Sphaerostomum bramae (Trematoda: Opecoelidae) wa
s carried out. The karyotype displays a typical 'plagiorchiid' pattern
with 22 chromosomes in a diploid set including a first pair of large
metacentric elements. Chromosomes with medially or submedially located
centromeres prevail in the karyotype; only chromosomes 7 and 9 are su
btelocentric. With the data now available, close karyological affinity
was noted between S. bramae, the representative of the family Opecoel
idae, and other karyologically studied species of some families in the
order Plagiorchiiformes: Troglotrematidae, Plagiorchiidae, Prosthogon
imidae, Lecithodendriidae and Telorchiidae.