EM18 AND EM16, NEW SEROLOGIC MARKER EPITOPES FOR ALVEOLAR ECHINOCOCCOSIS IN WESTERN-BLOT-ANALYSIS, ARE THE ONLY 2 EPITOPES RECOGNIZED BY COMMERCIALLY AVAILABLE WEAK POSITIVE (CUT-OFF) SERA FOR EM2(PLUS)-ELISA

Authors
ITO A OSAWA Y NAKAO M HORII T OKAMOTO M ITOH M YAMASHITA T
Citation
A. Ito et al., EM18 AND EM16, NEW SEROLOGIC MARKER EPITOPES FOR ALVEOLAR ECHINOCOCCOSIS IN WESTERN-BLOT-ANALYSIS, ARE THE ONLY 2 EPITOPES RECOGNIZED BY COMMERCIALLY AVAILABLE WEAK POSITIVE (CUT-OFF) SERA FOR EM2(PLUS)-ELISA, Journal of Helminthology, 69(4), 1995, pp. 369-371
Citations number
12
Categorie Soggetti
Zoology,Parasitiology
Journal title
Journal of Helminthology → ACNP
ISSN journal
0022149X
Volume
69
Issue
4
Year of publication
1995
Pages
369 - 371
Database
ISI
SICI code
0022-149X(1995)69:4<369:EAENSM>2.0.ZU;2-Q
Abstract
The assay system for antibody responses against Em2, the most specific antigen for serodiagnosis of alveolar echinococcosis (AE), has been e stablished by enzyme-linked immunosorbent assay (ELISA) but not by Wes tern blot assay, since Em2 antigen is not protein but carbohydrate in nature. Recently we reported that previously undescribed protein epito pes, designated Em18 and Em16 due to their molecular weights, were goo d serologic markers for AE by Western blot analysis. It has been shown that Em18 and Em16 are the only two epitopes recognized by commercial ly available weak positive (cut off) sera for the Em2(plus)-ELISA.