DETECTION BY PCR OF TOXOPLASMA-GONDII IN BLOOD IN THE DIAGNOSIS OF CEREBRAL TOXOPLASMOSIS IN PATIENTS WITH AIDS

The polymerase chain reaction (PCR) for amplification of Toxoplasma go ndii DNA was performed prospectively in the blood of 19 patients with AIDS and cerebral toxoplasmosis. The B1 gene and TGR1E sequence were u sed as targets and results were confirmed by hybridisation. Controls c onsisted of 24 HIV infected patients with tissue culture proven T gond ii parasitaemia and 57 HIV infected patients without toxoplasmosis. PC R was positive with both targets in 20 of 24 samples (84%) from patien ts with parasitaemia. Three of 57 samples (5%) from patients without t oxoplasmosis were PCR positive with either target, but none was positi ve with both targets. Only three of the 19 patients (16%) with cerebra l toxoplasmosis had a positive PCR with both targets before the start of specific treatment. PCR performed in blood is of little diagnostic value in cases of cerebral toxoplasmosis but could be useful in patien ts with disseminated infection.