EFFECT OF DENATURANTS ON THE SPECIATION OF AMINO-ACID COMPLEXES - COMPUTER AUGMENTED MODELING STUDIES .6. PROTONATION EQUILIBRIA OF L-GLUTAMIC ACID AND L-ORNITHINE IN ANIONIC L-ORNITHINE MEDIA

The effect of sodium lauryl sulphate(SLS) on the protonation equilibri a of L-glutamic acid and L-ornithine has been studied in varying conce ntrations (0.5-2.5% w/w) of SLS solution maintaining an ionic strength of 0.16 mol dm(-3) at 303 K. The protonation constants have been eval uated with the computer program MINIQUAD 75 from the primary alkalimet ric titration data. The selection of the best fit chemical models is b ased on chemical validation and residual analysis. All the three proto nation constants of L-glutamic acid decrease with increase in the SLS content. The same trend is observed for L-ornithine except for log K-3 which increased at higher concentrations of SLS. This trend is attrib uted to the changes associated with the dielectric constant of the med ium and to micellar effects.