PLATELET CAPACITY AND CRYOPRESERVATION

Loss of cells and morphofunctional performance of platelets were studi ed in cryopreservation of platelet concentrates (PC) at -196 degrees C under protection of dimethylsulfoxide (DMSO) using aluminum corrugate d and fluoroplastic disposable containers. PC were isolated by fractio nation of blood doses from plasma enriched with platelets. Cryoprotect ion was conducted with protective solution 10% DMSO in autoplasm. Free zing followed by storage in liquid nitrogen proceeded according to the program from 1 degrees C/min to -60 degrees C. In preservation using aluminum containers cell losses upon defrosting came to 16%, after was hing 35%, the ability to respond to hypotonic stress averaged 55% comp ared to that before freezing. Cryopreservation in polymeric containers reduced cell losses to 3% after defrosting and to 25% after washing, the response to hypotonic stress made up 80% from the initial value. P C cryopreservation in liquid nitrogen with DMSO allows satisfactory qu antitative and morphofunctional safety of the cells. The technique of freezing in polymetic containers is preferable as regards cell safety.