The trachealess (trh) gene of Drosophila is required for embryonic tub
e formation. In trh mutants, tube-forming cells of the salivary gland,
trachea, and filzkorper fail to invaginate to form tubes and remain o
n the embryo surface. We identified a P-element insertion that disrupt
s trh function and used the insert to clone and characterize trh. trh
is expressed in the salivary duct, trachea, and filzkoper primordia, a
nd expression persists in these cells throughout embryogenesis. trh ex
pression in the salivary duct is controlled by the homeotic gene, Sex
combs reduced (Scr), and by another salivary gland gene, fork head (fk
h). trh is homologous to two transcription factors: the human hypoxia-
inducible factor-1 alpha and the Drosophila Single-minded protein.