We found naturally occurring, potentially virulent Escherichia coli O1
57:H7 strains in sheep. The incidence of E. coli O157:H7 was transient
and ranged from 31% of sheep in June to none in November. The use of
a sensitive culture technique and the choice of the proper sampling se
ason were both essential for detecting this bacterium in sheep. DNA hy
bridizations showed that 80% of the E. coli O157:H7 isolates had at le
ast two of the Shiga-like toxin types I or II or the attaching-effacin
g lesion genes.