QUANTITATING STAPHYLOCOCCAL-ENTEROTOXIN-B IN DIVERSE MEDIA USING A PORTABLE FIBEROPTIC BIOSENSOR

A new, portable fiber-optic biosensor has been used to detect staphylo coccal enterotoxin B, a causative agent of food poisoning, at levels a s low as 0.5 ng/ml in buffer. The toxin (SEE) can also be detected and quantitated in other relevant media: human serum, urine, and aqueous extract of ham. The level of toxin, from 5 to 200 ng/ml, can be accura tely predicted in these media by calibrating each fiber and by compari ng results to a single standard curve based on toxin in buffer. The qu antitative fluorescent sandwich immunoassay provides results in 45 min ; qualitative results are provided in 15-20 min, Using a blender and a benchtop centrifuge, fast, simple aqueous extracts of contaminated ha m samples were prepared and tested. Ham spiked with 5 or 40 mu g SEE p er 100 g food resulted in biosensor readings indicative of 11 or 69% r ecovery of the toxin, respectively, Finally, the SEE assay is highly s pecific; SEA and SED give only 2-3% of the signal at 5000 ng/ml as SEE gives at 1000 ng/ml. This specific, sensitive assay for SEE on the po rtable fiberoptic biosensor permits easy monitoring of clinical sample s or on-site analysis of suspect food samples. (C) 1996 Academic Press , Inc.