MURINE AND HUMAN MAST-CELL EXPRESS ACETYLCHOLINESTERASE

Expression of catalytically active protein was detected in a murine ma st cell line, The primary type of AChE mRNA produced by these cells wa s found to be the brain and muscle type by PCR amplification of altern ative exons from the 3' of mast cells AChE cDNA. AChE was further foun d to be expressed in the HMC-1 the human mast cell precursor line, Fur thermore, utilizing the single cell RT-PCR method we detected AChE mRN A expression in Fc epsilon RI-positive single cells derived from human colonic mucosal biopsies. Our findings predict the involvement of mas t cell. AChE in neuronal-mast cell interactions.